Detection of heavy metal and antibiotics resistant in some isolates of Bacillus isolated from Iraqi soil

  • Al- Khafaji, K. A. Genetic Engineering and Food Biotechnology Center, Agricultural and Food Technology Directorate, Ministry of Science and Technology, Baghdad, Iraq.
  • Saffa A. R. Genetic Engineering and Food Biotechnology Center, Agricultural and Food Technology Directorate, Ministry of Science and Technology, Baghdad, Iraq.
  • abid al- Hadi Genetic Engineering and Food Biotechnology Center, Agricultural and Food Technology Directorate, Ministry of Science and Technology, Baghdad, Iraq.
Keywords: heavy metals, B. alcalophilus, B. stearothermophilus, plasmid.

Abstract

Heavy metals and antibiotic resistance were screened for local isolates of Bacillus species which either alkalophil or thermophil. Differences in their heavy metals resistant were detected against Pb, Hg, Cr, Co, Mo and Zn. Antibiotic resistant for ampicillin, cholaramphenicol, tetracycline, kanamycin, rifampcin and streptomycin were detected. Some of the isolates belong to B. alcalophilus have heavy metals and antibiotic resistance. DNA electrophoresis showed difference in plasmids size and electrophoresis profile of some isolates of B. alcalophilus, no plasmid was recognized in DNA samples of B. stearothermophilus.

Published
2016-06-30
How to Cite
[1]
Al- Khafaji, K. A., Saffa A. R., and abid al- Hadi, “Detection of heavy metal and antibiotics resistant in some isolates of Bacillus isolated from Iraqi soil ”, JMAUC, vol. 8, no. 1, pp. 5-13, Jun. 2016.
Issue
Vol 8 No 1 (2016)
Section
Articles