Isolation and Identification Salmonella ssp from Local and Imported Chicken Meat by using VIDAS® -Up Salmonella
This study was carried out to isolate Salmonella species from Chicken meat, comparing the conventional method with rapid methods VIDAS ® -Up salmonella SPT (BioMérieux), 142 meat samples (Chicken) from the Local and Imported meat From local markets in the city of Baghdad within the period Septembers 2013 -march 2014. nineteen isolates were diagnosed as genus Salmonella and ensured by National center of Salmonella in Baghdad. nineteen Salmonella serotype (15.8 %) were diagnosed from chicken meat that includes S.typhimurium (% 31.5) S.braenderup, S. dublin S. antum, and S. hadar ( 10.5 % ) for each of them and finally S.entertidis , S.livingstone and S.give at 5.2 % for each of them . The study recorded the serotypes of S.give and S. living stone as the first time in Iraq from chicken meat These results showed high significant differences at (P <0.01) between food samples. The results revealed that the contamination of imported meat was more than local meat and recorded of Salmonella isolates from imported meat that higher compared with local meat , The results Sensitivity test eight antibiotics (Ampicillin , Amoxycillin , cefitizdime, Chloramphenicole ,Impinem, trimethprime, Ciprofloxacin and Ceftriaxone) were observed resistant of the serotypes isolated from food samples towards Trimethprime Cetazidime, Ampicillin, Chloromphenicol, Ceftriaxone, and Amoxicillin at (100 , 86.6 ,40,30 , 33.3 and 16.6 %) respectively, and showed that the sensitivity for each of Imipenem and Ciprofloxacin 100%. VIDAS ® UP Salmonella showed high values of specificity and accuracy. VIDAS ® significantly decreased the time to obtain results compared to the conventional method and this is an important factor in the selection of an analytical method.