Optimum Conditions of Protease Production from Bacillus licheniformis (B1) and its Applications

  • Haider Jawad Kadhim Biology Department, College of Science, University of Baghdad
  • Sanaa Burhan Aldeen Biology Department, College of Science, University of Baghdad.
Keywords: Protease, Production, Bacillus licheniformis, applications.

Abstract

Forty isolates of Bacillus spp. were isolated from sixty samples including; soil, water and meat. Ability of these isolates to produce protease was evaluated. Bacillus B1 isolated that isolated from soil showed the highest protease production. it was identified as a strain of Bacillus licheniformis.The optimum culture medium and conditions for protease production were casein-yeast extract medium contained soluble casein (0.5g), yeast extract (0.5g), glucose (1g), KH2PO4 (0.02g), K2HPO4 (0.02g) and MgSO4.7H2O (0.01g), in 100ml distilled water, pH 8.0 and incubated at 37˚C. for 48 h. The crude protease exhibited ability to remove the blood color from cloth within 30 min and gelatin from X-ray film within 120 min. 

Published
2014-06-30
How to Cite
[1]
Haider Jawad Kadhim and Sanaa Burhan Aldeen, “Optimum Conditions of Protease Production from Bacillus licheniformis (B1) and its Applications ”, JMAUC, vol. 6, no. 1, pp. 26-39, Jun. 2014.
Issue
Vol 6 No 1 (2014)
Section
Articles