Spectrophotometric Determination of Chloramphenicol in Bulk and Pharmaceutical Preparation

Tariq Y. Mahmoud; Sarmad B. Dikran; Alaa K. Mohammed

Tariq Y. Mahmoud Dept. of Chemistry- College of Education for Pure Science (Ibn Al- Haitham)/ University of Baghdad
Sarmad B. Dikran Dept. of Chemistry- College of Education for Pure Science (Ibn Al- Haitham)/ University of Baghdad
Alaa K. Mohammed Dept. of Chemistry- College of Education for Pure Science (Ibn Al- Haitham)/ University of Baghdad

Keywords: chloramphenicol determination, spectrophotometry,central composite experimental design.

Abstract

Chloramphenicol is spectrophotometrically determined by the formation of a colored charge transfer exhibiting λmax at 445 nm after the reaction of the secondary amine with sodium nitroprusside in the presence of NH2OH in alkaline medium. Classical univariate and chemometric central composite experimental design approaches were used to find the optimum experimental conditions for the parameters affecting the formation of CT-complex. The proposed method is simple and sensitive for the determination of the drug in a concentration rage of 1.0-25.0 μg.mL-1 with molar absorptivity8.142 x 103L.mol-1.cm. and r= 0.9995. The validity of the method was confirmed by finding the regression equation, accuracy, precision, and detection limit. Chloramphenicol was successfully determined in its pharmaceutical preparations by the developed procedure with a reasonable of recovery and precision. Statistical validation of the obtained results was made and the method shows acceptable recovery and repeatability.